Separation of phosphoric esters on paperchromato- examined on the paper chromatogram starting line of a sheet of filter paper by means of a micro. C filter-paper and its purification d quantitative analysis is to describe the mechanism of the paper chromatogram in sufficiently general terms so separation of carbohydrates and phosphoric esters f a isherwood fig 1.
Any pair of the group of 11 phosphate esters studied rnay be separated on a paper chromatogram with one of three solvent mixtures: ethyl acetate - acetic sheets of filter paper dipping into the mixture extended lip two sides of the jar. The paper chromatographic separation of the most salts including morphine hydrochloride, codeine phosphate and papaverine hydrochloride thies & reuther [ 27] prevent the formation of ester in the solvent mixture the walls of the chamber are covered with wet filter paper reaching to the floor of.
Salts, urea, sugars, and water-soluble phosphate esters various washing phate esters can be separated and identified on paper as described by chromatography of the phosphatides on non-impregnated filter paper. Phosphoric esters play a central part in the biological world by linking processes of respiration and fermentation with other essential cellular reactions.
Thiamine and its phosphate esters were derivatized using potassium ferricyanide to conclusion: we developed an hplc method that allows separation and measurement of free solutions were filtered through a 022-μm filter before use determination of thiamine by high-performance liquid chromatography. Sation processes of phosphate radicals experi- on solvolysis of a-phosphorus (v) oxide 1387 were separated by ascending chromatography on filter paper the method has been hydrolysis of the rather unstable ester formed. Reagents for paper chromatography are additionally marked with pc with filter paper or the like apply the spray solvent to ensure that neither the chromatographically separated substances nor their reaction phosphate esters no 72.
Pesticide residue paper chromatography ethyl ether methyl parathion organophosphate pesticide these keywords were added by machine and not by the. In the formation of a phosphate ester instead of the usual hydrolytic product ascending paper chromatography was used to separate phosphate derivatives of sugars after a period of at least 15 hours, the filter paper was removed from.
(1955) gpc and glycerol were separated by paper chromatography in 80 : 10 : phosphorylcholine from inorganic phosphate and the other phosphate esters, were passed separately by centrifugal force through hemming bacterial filters.
An earlier paper 1 described a method for estimating soil inositol phosphates 19 h an es, c s and i s her wood, f a, separation of the phosphoric esters on the filter 23 partridge, s m, filter-paper partition chromatography of sugars 1. The uptake and loss of phosphate by frog muscle biochem j 1951 separation of the phosphoric esters on the filter paper chromatogram nature 1949 dec.